RNA-Seq of yeast - SRA

SRX3777941: RNA-Seq of yeast
1 ILLUMINA (Illumina HiSeq 2000) run: 16.7M spots, 5G bases, 1.8Gb downloads

Design: Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT) by binding A-T to the ployA tail of the mRNA. Subsequently, break the mRNA into short fragments. The mRNA was used as a template to synthesize a strand of cDNA with six random bases and then the buffer, dNTPs and DNA polymerase I were used to synthesize the double-stranded cDNA. AMPure XP beads were then added to purify double-stranded cDNA. PCR-enrichment was performed to obtain the final cDNA library.

Submitted by: Northwest A&F University

Study: transcriptome

PRJNA437612 • SRP134252 • All experiments • All runs

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This work presents a combination of experiment and analysis aimed to describe the priority tolerant ability of Z. rouxii which caused food spoilage. These knowledge will provide clues or methods to decrease the food spoilage caused by sugar and salt resistant-yeast species.

Sample:

SAMN08668440 • SRS3031790 • All experiments • All runs

Organism: Zygosaccharomyces rouxii

Library:

Name: Z_Stress2

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: PAIRED

Runs: 1 run, 16.7M spots, 5G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR6821095	16,663,744	5G	1.8Gb	2018-03-09

ID:: 5208486

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